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KMID : 0903620010420040399
Journal of the Korean Society for Horticultural Science
2001 Volume.42 No. 4 p.399 ~ p.404
Effect of Temperature of Irrigation Water on the Growth and Activities of Some Enzymes in Cucumber Seedling (Cucumis sativus L.)



Abstract
This study was conducted to investigate the growth and activities of some isozymes in cucumber seedlings grown with irrigation water of different temperatures. The objectives of this study were to determine: growth in plant height, number of leaves and leaf area at 35 days after sowing; and activities of some isozymes related to the adaption to environmental stresses at 21 days after sowing. Plant height, number of leaves and leaf area were influenced by temperature of irrigation water. Growth rate of seedlings grown with water temperature of 25¡¾2¡É was greater as compared with 13¡¾2¡É. Root and total fresh weights were not affected. Activities of catalase, guaiacol peroxidase and ascorbate peroxidase in the leaves with 25¡¾2¡É water irrigation were lower than those of 13¡¾2¡É, whereas activity of glutathione reductase was not affected. Gels stained for guaiacol peroxidase and glutathione reductase showed three isoforms, and the isoform of ascorbate peroxidase was one. The intensities of active bands in each treatments were in accordance with the induction profiles of peroxidase and glutathione reductase specific activity.
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